Bacterial Smear Preparation

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  • Опубликовано: 12 апр 2025

Комментарии • 58

  • @ericagovea9203
    @ericagovea9203 Год назад +4

    Why aren’t you using any gloves ? Is it PPE not required ?

  • @kenlordnavarette8734
    @kenlordnavarette8734 4 года назад +6

    What do you think would be easier to prepare bacterial smear from, liquid or solid culture?

    • @abrarbhatti8978
      @abrarbhatti8978 2 года назад

      Send your whattsapp number

    • @mood.my202
      @mood.my202 Год назад

      هل وجدت الجواب؟

    • @L4v4molly
      @L4v4molly 11 месяцев назад +2

      It's easier to obtain colonies separately from solid media. It's also more likely to carry unnecessary parts from the liquid media.

  • @yeppy013
    @yeppy013 6 лет назад +1

    What is the purpose of air drying before heat fixing? Can't the heat fix do that as well as to dry the slide quicker?

    • @angelaasencion397
      @angelaasencion397 6 лет назад +2

      The water will boil out and the bacteria will be destroyed.

    • @eclip9
      @eclip9 6 лет назад +2

      Heat damage them so you need to be careful already you can dry it on the flame but with keeping a good distamce in order to prevent it from getting hot

    • @isiomawizzy3669
      @isiomawizzy3669 Год назад

      Air drying preserves the morphology of the cell but heating distorts it.

  • @sicelogemane9249
    @sicelogemane9249 4 года назад

    Why the alcohol is used to clean the slide and not water?

  • @ayatosakamaki92
    @ayatosakamaki92 3 года назад +2

    I'm confused , is the first drop of water compulsory???

    • @rayayn81
      @rayayn81 2 года назад +1

      Water is required only if its a solid culture from a plate but not for broth cultures

    • @freefirebluezone
      @freefirebluezone Год назад

      ​@@rayayn81thnku

  • @DatChoklateGurl69
    @DatChoklateGurl69 8 лет назад +37

    why didn't you have on gloves?

    • @iradukundasylvie1148
      @iradukundasylvie1148 6 лет назад +2

      I guess it's not necessary

    • @cassiloca
      @cassiloca 6 лет назад +1

      you don't need them

    • @kvnkvn9191
      @kvnkvn9191 6 лет назад +4

      Not necessary because culture is created so no contamination chances in sile by hand

    • @ramkay9706
      @ramkay9706 6 лет назад +9

      youre dealing with fire, glove is not necessary

    • @fluffandstuff6089
      @fluffandstuff6089 4 года назад +1

      Could cause a burn if the glove melts on your hand

  • @althehore20
    @althehore20 3 года назад +1

    Good ❤

  • @epic11introvert77
    @epic11introvert77 3 года назад

    इस there bacteria in water and air will not affect it

  • @maryamadarbeh2323
    @maryamadarbeh2323 5 лет назад +1

    Why it’s important to air dry the smear before heat fixing?

    • @tayelorg8605
      @tayelorg8605 4 года назад +7

      Hi, I'm a microbiology student in my second year. If the sample isn't dry first you are likely to boil the bacteria instantly and all your cells may lyse destroying them.

  • @LifeWithLonwabo
    @LifeWithLonwabo 2 года назад +3

    why do we heat the mouth of the tube

    • @wetherly
      @wetherly 6 месяцев назад +1

      Creates a hot convection current sterilizing the air and pushing out any unwanted microbes. Gotta keep the tube sterile at all times too for purity sake

  • @beomnyang
    @beomnyang 3 года назад

    good job susan

  • @محمدشاكرفليححسن.2
    @محمدشاكرفليححسن.2 4 года назад +1

    good

  • @edidiongekpo
    @edidiongekpo 6 лет назад +1

    Great video!

  • @lister2504
    @lister2504 4 года назад

    Good video

  • @noorjahan2246
    @noorjahan2246 4 года назад

    What she took from test tube??

  • @user-wk2gp3tu8h
    @user-wk2gp3tu8h 3 года назад +1

    Thank you ❤️😊💯

  • @zuluzero4659
    @zuluzero4659 6 лет назад +4

    Where is ur lab coat where is your gloves?

    • @CynicalDuty
      @CynicalDuty 6 лет назад +1

      chase the bag, don't worry about what i'm doing

    • @zuluzero4659
      @zuluzero4659 6 лет назад

      @@CynicalDuty yes but your contaminating the results so thats wrong. Your also a threat to your health. I have got my youtube money hun x there is a protocol and h&s coshh for a reason if you work for any creditied lab or school and see you doing this, you will get reprimanded.

    • @CynicalDuty
      @CynicalDuty 6 лет назад +2

      @@zuluzero4659 bust down, thotiana

    • @zuluzero4659
      @zuluzero4659 6 лет назад

      @@CynicalDuty Uh, ayo, I been a bad b*tch, cockiana (Cockiana) / And I'm still winnin', Pacquiana (Pacquiana) / Percocets, popiana, killin' everybody beat / Thank you next, Ariana (Ariana)

    • @tayelorg8605
      @tayelorg8605 4 года назад

      @@zuluzero4659 I'm not too sure which school you are referring to but at Curtin in Perth, in second-year Microbiology we don't use gloves. It is optional. As for the Lab coat yes it is standard PPE and we all need one. The results are not contaminated by bacteria on our hands nor can the bacteria on the stain infect us as it has been made bacteriostatic from use of the gram staining reagents. The bacteria on the slide are in too significant number to be contaminated by our own natural flora. Note also how they have been heat fixed first so our natural flora would just be washed off the slide if it was present.

  • @MohammedAyaanQureshi
    @MohammedAyaanQureshi 3 года назад +1

    Thankyou. Doc

  • @jesslynwong4666
    @jesslynwong4666 11 месяцев назад

    NO GLOVES ?! OH NO

  • @SaraAli-cl8qs
    @SaraAli-cl8qs 4 месяца назад

    Perfect

  • @chandannishad4167
    @chandannishad4167 4 года назад +1

    Vedasi mam ki aaesi ki tasi . 😁😁👍

  • @Oh-ou4lp
    @Oh-ou4lp 6 лет назад

    ok where is the rest

  • @Hu_Ns1
    @Hu_Ns1 2 года назад

    thik smear
    With out gloves
    🤔

  • @senaityemanne422
    @senaityemanne422 6 лет назад +1

    No gloves?

  • @2009abody
    @2009abody 7 лет назад

    Thank you

  • @asmajahan6079
    @asmajahan6079 7 лет назад

    Thax dea

  • @phali6253
    @phali6253 4 года назад

    سلام الله على الله اذا افتهمت شي 😂🤦

  • @brunoasavaz8773
    @brunoasavaz8773 2 года назад

    「コンテンツを調整する必要があります」、

  • @chandannishad4167
    @chandannishad4167 4 года назад +1

    Samaj Nahi aarha hai .
    English girl'.
    Jara ap Hindi me batiya . Ok
    🍻😝😝😝